1. Sand pit route

2. Successional site in the Cep I sandpit

This is a succesional site (6 ha: around 500 × 120 m, 48°55‘4“N, 14°52‘60“E) located within the large mostly flooded sand pit (ca.140 ha) situated in the southern part of the Třeboňsko Protected Landscape Area (40 km SE from České Budějovice). After the end of mining in the middle of the 1990s the restoration scheme was rearranged and the site was left to spontaneous development. In 2002 – 2006, the surface was modulated and shallow lakes were created. Communities of bare sand and oligotrophic wetlands gradually established in the site. Slopes at the edge of the site are afforested by Scots Pine (Pinus sylvestris) and English Oak (Quercus robur) as a compromise between near-natural and traditional forest reclamation. Recently, spontaneous succession includes wetland communities in a diverse mosaic with sandy communities. Sandy banks are used by nesting Little Ringed Plover (Charadrius dubius) and Sand Martins (Riparia riparia).

4. Cep II sand pit and the Klara's island

Klara's island (0.3 ha: 70 x 40m, 48°55'59"N, 14°52'39"E) is located in the southeastern part of the large sand pit (101 ha). Mining of the sand pit Cep II started in 1979. A majority of the area is formed by a large lake formed by extraction of gravel-sand below the water level. Application of near-natural restoration methods by the Administration of the Třeboňsko Protected Landscape Area led to the decrease of technical reclamation in the extracted areas since the end of the 1990s. The recent restoration scheme assumes creation of a gentle lake coastline (including Klara's island) with favourable conditions for shallow water vegetation.  Slopes at the edge of the site are afforested by Scots Pine (Pinus sylvestris) and English Oak (Quercus robur) as a compromise between near-natural and traditional forest reclamation. Several rare amphibians and reptiles live in the sand pit, e.g. a large colony of Little Ringed Plover (Charadrius dubius).

A part of the extracted material was deposited in the southern part of the lake to create the island in 2005. In 2009, Klara's island was used in an experimental trial to restore very fragmented psammophytic grasslands on suitable new sites such as abandoned sand pits.

9. Červené blato peatland

The locality is situated in the Třeboňsko Biosphere reserve (South Bohemia). It is one of the largest raised bogs in the lower altitutes (470 m a.s.l.) with bog pine (Pinus rotundata) in the Czech Republic. Because the drainage channels were functioning still after almost 100 years, dams were installed to prevent further draining of the site.

The vegetation represents a mosaic of different successional stages formed by earlier block-cutting of peat. The open habitats are dominated by cotton grass (Eriophorum vaginatum) and sphagnum mosses (S. fallax, S. cuspidatum). Other typical bog species occur in the bog forest, with some glacial relicts in the flora and fauna such as Ledum palustre, Oxycoccus palustris, Andromeda polifolia, and Drosera rotundifolia. The locality is rich mainly in invertebrates, many species of dragonflies (i.g. Aeshna subarctica, Leucorrhinia rubicunda), butterflies (i.g. Arichanna melanaria), and beetles (i.g. Lestus terminatus, Agonum ericeti).

The block-cut site is accesible to visitors via a nature trail which was built there by the Authority of the Třeboňsko Biosphere Reserve.

18. Třeboň, a historical town

The town, protected by national law, was founded in the 13th century and flourished especially in the 16th century. The centre is well preserved as a whole, with a beautiful central square, a large renaissance castle and a valuable gothic church with an attached monastery. The entrance to the town is possible only through the four historical gates. The town is located in the centre of the extensive fishpond system built mostly in the 16th century.